No anticoagulants, or preservatives should be added. Anti-Desmoglein-1 (DSG1) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues. Materials and reagents Expand Equipment Leica CM1950 automatic sectioning cryostat.Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5).Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass. products categories : Cell Biology. Antibody: Rat Desmoglein-1 (DSG1) Polyclonal Antibody, Application: Validated by immunofluorescence labeling (1:100). Human Desmoglein­1 Monoclonal Antibody (Catalog # MAB944) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti­Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). VAT, excl. Patients with pemphigus vulgaris, a deeper form of pemphigus, have antibodies to DSG3 and sometimes DSG1 as well. Desmoglein 1 is a desmosomal cadherin with a molecular weight of 160kD, whereas desmoglein 3 has a molecular weight of 130kD. The diagnosis of pemphigus depends on biopsy and serum studies that characterize lesions and … Immunogen Desmoglein-1 Precursor recombinant protein epitope signature tag (PrEST) Application All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org) and as a result, are supported by the most extensive characterization in the industry. Desmogleins, along with desmocollins, are cadherin-like transmembrane glycoproteins that are major components of the desmosome. Desmoglein-1 is a member of the desmoglein protein subfamily. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (ab124798) ab124798, at a 1/250 dilution, staining Desmoglein 1/DSG1 in … Design: Retrospective study. Like other cadherins, desmoglein-1 contains multiple calcium binding sites and a cell-adhesion recognition site which facilitates intercellular adhesio… Reconstructed human epidermis tissues were embedded in paraffin. Cat. Desmoglein 1 Antibodies . Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. General Protocols Anti-Desmoglein 1 antibodies are available from several suppliers. No. Desmoglein-1 is a member of the desmoglein protein subfamily. The severity of cutaneous and oral pemphigus is related to desmoglein 1 and 3 antibody levels. Specific staining was localized to keratinocyte membranes. Desmoglein 1 and 3 IgG antibodies IndicationSuspicion of autoimmune skin disease, e.g. Br J Dermatol. Desmoglein-1 (DSG1) is a transmembrane glycoprotein belonging to the family of cadherins. Antibody titer correlates in a semiquantitative manner with … Anti-Desmoglein 1/DSG1 antibody [EP6765] (ab133662) at 1/1000 dilution + Human fetal skin lysate at 10 µg Secondary HRP conjugated Goat anti-Rabbit at 1/2000 dilution Predicted band size: 114 kDa. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Upon receipt, store it immediately at the temperature recommended below. Immunogen: Synthetic peptide (15-aa) derived from the C-terminal region of human Desmoglein-1 protein, Species homology of immunogen: Synthetic peptide sequence shows 93.3% homology to mouse or rat sequence, Storage buffer: PBS, pH 7.2, 0.1% sodium azide. Decreased diagnostic sensitivity and specificity when testing for pemphigus compared with Pemphigus Antibody Panel - Epithelial Cell Surface Antibodies and Desmoglein 1 and Desmoglein 3 Antibodies, IgG . Excl. pemphigus and differential diagnosis of pemphigoid, EBA etc.MethodELISA with recombinant antigens desmoglein 1 and 3. Enzyme Linked Immunosorbent Assay (ELISA) Methodology. A 1:100 dilution of the antibody was used (overnight incubation at 4°C). Antibodies to desmoglein 1 (DSG1) and desmoglein 3 (DSG3) have been shown to be present in patients with pemphigus. Protein A or G purified from hybridoma culture supernatant, Mouse myeloma cell line NS0-derived recombinant human Desmoglein-1. Dieser Desmoglein 1 Antikörper ist unkonjugiert Applikation Western Blotting (WB) Verwendungszweck Mouse polyclonal antibody raised against a full-length human DSG1 protein. To better understand PF pathophysiology, we used phage … In turn, these proteins link the Desmoglein-1 with the intermediate filaments. Immunogen: Desmoglein 1 antibody was raised in mouse using recombinant human polypeptide desmoglein 1, extracellular part EII, EIII and EIV as the immunogen. Allow the blood to clot at room temperature (20-25° C) and separate as soon as possible to limit hemolysis. Desmoglein‑1 in Human Skin Hyperplasia. Setting: Dermatology departments from 13 university hospitals in France. ncbi gi num : 119703744. ncbi acc num : NP_001933.2. Desmosomes are cell-cell junctions between epithelial, myocardial and certain other cell types. The desmoglein-1 subtype is highly expressed in the epidermis. Desmoglein‑1 in Human Skin Hyperplasia. ncbi mol weight : … As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally. Together with desmocollins, another subfamily within the cadherin superfamily, the desmoglein isoforms form the adhesive components of desmosomes, the cell-cell adhesive structures that are found in epithelial cells. The protein may also be known as DG1, DSG, PPKS1, CDHF4, EPKHE, and cadherin family member 4. Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. The protein encoded by this gene has been identified as the autoantigen of the autoim… Desmoglein versus non-desmoglein signaling in pemphigus acantholysis: characterization of novel signaling pathways downstream of pemphigus vulgaris antigens. The data collected includes not only links to publications in PubMed, It differs from other classic cadherins by having four instead of five cadherin repeat domains in its extracellular region, and a much larger cytoplasmic region containing five desmoglein repeat domains which share homology with the cadherin repeats. Marke MaxPab® Kreuzreaktivität Human Immunogen. Customer Support: customercare@bicellscientific.com, Technical Issues: tech@bicellscientific.com, Validated Antibodies – The Foundation of Biomedical Research. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. Many patients with pemphigus foliaceus, a superficial form of pemphigus have antibodies to DSG1. Antibody titer correlates in a semiquantitative manner with … Filter your results: No product specific FAQs exist for this product, however you may, Average Rating: 4.5 [email protected] Buffers Expand BLOCK Solution  NaCl 8 gKCl 0.2 gNa2HPO4 1.44 gKH2PO4 […], Antibody: Rabbit VE-cadherin (Cdh5 or Cadherin-5 or CD144) Polyclonal Antibody, Antibody: Rabbit Anti-Claudin-10 (CLDN10) Polyclonal Antibody, Antibody: Rabbit Anti-ZO-1 (Tjp1) (N-terminus) polyclonal antibody. Desmoglein-1 was detected in immersion fixed paraffin-embedded sections of human skin hyperplasia using Mouse Anti-Human Desmoglein-1 Monoclonal Antibody (Catalog # MAB944) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). We offer a 100% guarantee on all our products. The product is shipped at ambient temperature. © Copyright 2021 R&D Systems, Inc. All Rights Reserved. Many patients with pemphigus foliaceus, a superficial form of pemphigus have antibodies to DSG1. Avoid using sera exhibiting a high level of lipemia, hemolysis, icterus or microbial growth. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. *Small pack size (SP) is shipped with polar packs. Desmoglein-1 is one of three members of the desmoglein subfamily of calcium-dependent cadherin cell adhesion molecules. Currently, four desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. Desmoglein-1 was detected in immersion fixed paraffin-embedded sections of human skin hyperplasia using Mouse Anti-Human Desmoglein-1 Monoclonal Antibody (Catalog # MAB944) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS. Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (ab124798) at 1/1000 dilution + Human skin lysate at 10 µg Secondary HRP labelled goat anti-rabbit at 1/2000 dilution Predicted band size: 114 kDa. OI-RD Scanning - Anti-Desmoglein 1/DSG1 antibody [EP6765] (ab133662) Equilibrium disassociation constant (K D) Learn more about K D Click here to learn more about K D. Protocols. Desmogleins, along with desmocollins, are cadherin-like transmembrane … Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR.Tissue-tek cryomolds from VWR. Human Desmoglein-1 is a type I transmembrane glycoprotein of 1049 amino acid (aa) residues with a 23 aa signal peptide and a 26 aa propeptide. 1 month, 2 to 8 °C under sterile conditions after reconstitution. Desmoglein‑1 in Human Skin Hyperplasia. uniprot acc num : Q02413. Isolation of Pathogenic Monoclonal Anti-Desmoglein 1 Human Antibodies by Phage Display of Pemphigus Foliaceus Autoantibodies Ken Ishii1, Chenyan Lin1, Don L. Siegel2 and John R. Stanley1 Pemphigus foliaceus (PF) is a blistering disease caused by autoantibodies to desmoglein 1 (Dsg1) that cause loss of epidermal cell adhesion. Antibody dilution 1:50, works also at 1:100 dilution. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest. Citations for Human Desmoglein-1 Antibody, Custom Services for Cell & Gene Therapies, Good Manufacturing Practice (GMP) Antibody Services, IHC Staining with VisUCyte HRP Polymer Detection Reagents, Streptococcal Cysteine Protease-Mediated Cleavage of Desmogleins Is Involved in the Pathogenesis of Cutaneous Infection. The expected protein mass is 113.7 kDa, but there are 2 reported isoforms. Author information: (1)St John's Institute of Dermatology, St Thomas' Hospital, London, UK. It is one of the main structural components of desmosomal junctions which are abundant in tissues undergoing frequent mechanical stress, such as the heart, epidermis, lymphatic endothelial cells, and meninges. Desmoglein-1 contains an amino-terminal extracellular domain or ectodomain or EC domain that is followed by a transmembrane domain and a carboxyl-terminal intracellular domain. Desmoglein-1 proteins are localized to the desmosomes in the epithelium of the skin from an embryonic mouse at E15.5. Circulating IgG antibodies to desmoglein-1 and -3 (Dsg1, Dsg3) adhesion molecules of squamous epithelial cells are the underlying defects, causing loss of cell-to-cell adhesion. The diagnosis of pemphigus depends on biopsy and serum studies that characterize lesions and … Desmoglein-1, also known as Cadherin family member 4 (CDHF4), is encoded by the DSG1 gene in human. WHERE SCIENCE INTERSECTS INNOVATIONTM. Contact us for questions or custom requests! Desmoglein-1 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. These antibodies bind to calcium dependent adhesion molecules in cell surface desmosomes, notably desmoglein 1(DSG-1) in pemphigus foliaceus and desmoglein 3 (DSG-3) in pemphigus vulgaris. Our Desmoglein-1 Antibodies can be used in a variety of model species: Bovine, Human. None found for this test Please visit our Clinical Education Center to stay informed on any future publications, webinars, or other education opportunities. These antibodies bind to calcium dependent adhesion molecules in cell surface desmosomes, notably desmoglein 1(DSG-1) in pemphigus foliaceus and desmoglein 3 (DSG-3) in pemphigus vulgaris. Patients with pemphigus vulgaris, a deeper form of pemphigus, have antibodies to DSG3 and sometimes DSG1 as well. Reconstitute at 0.5 mg/mL in sterile PBS. Bio-Techne Harman KE(1), Seed PT, Gratian MJ, Bhogal BS, Challacombe SJ, Black MM. For Research Use Only. Each Desmoglein-1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Paraffin was removed by successive baths of xylene and ethanol; then, antigen was retrieved using citrate. are available in the Technical Information section on our website. Please Note: Optimal dilutions should be determined by each laboratory for each application. Objective: To assess the predictive value of anti-desmoglein (Dsg) 1 and anti-Dsg3 antibody (Ab) enzyme-linked immunosorbent assay (ELISA) values for the occurrence of relapses in pemphigus. These desmoglein gene family members are located in a cluster on chromosome 18. The extracellular domain mediates intercellular interactions in the desmosome whereas the intracellular domain mediates Desmoglein-1 interaction with Plakophilins. A direct immunofluorescence test showed intercellular space deposits of IgG, and the ELISA index to anti-desmoglein 1 antibodies was raised to 115 (reference range <20). Anti-Mouse HRP-DAB Cell & Tissue Staining Kit, 19 Citations, VisUCyte Antigen Retrieval Reagent-Universal, VisUCyte Antigen Retrieval Reagent-Acidic, 2 Citations, 13 Citations, 8 Citations, Antigen Retrieval Reagent Sampler (50 mL each), 7 Citations, 1 Image, 42 Citations, Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody, 12 Citations, Rat Anti-Mouse IgG2A PerCP-conjugated Antibody, 56 Citations, Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody, Rat Anti-Mouse IgG2A PE-conjugated Antibody, Goat Anti-Mouse IgG Biotinylated Antibody, 4 Citations, Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody, Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody, 5 Citations, Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody, 6 Citations, Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody, 1 Citations, 2 Images, Rat Anti-Mouse IgG2A APC-conjugated Antibody, Donkey Anti-Mouse IgG Biotinylated Antibody, 11 Citations, Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody, Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab, 3 Citations, Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody. ncbi gb acc num : NM_001942.2. Anti-Desmoglein-1 (DSG1) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues. Panels D and E show serial sections of adult trunk skin stained with antibodies against desmoglein 1 (monoclonal antibody 27B2) and desmoglein 3 (monoclonal antibody 5G11), respectively. Desmoglein-1 has been shown to be important in establishing cell-cell adhesion and function in the epidermis. The cytoplasmic tail of Desmoglein-1 interacts with desmoplakins, plakoglobin and plakophilins. Desmoglein Antibodies (1 and 3) Test Resources. R&D Systems personnel manually curate a database that contains references using R&D Systems products. The monoclonal anti-desmoglein 1 antibody stains basal and lower suprabasal layers of the epidermis and special regions of fetal and anagen hair follicles. Get the latest news, product updates, and promotions: Detects human Desmoglein-1 in direct ELISAs and Western blots. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Consider ordering with Epithelial Cell Surface Antibody IgG . 2 immunogen: DSG1 (AAI53002.1, 1 a.a. ~ 1049 a.a) full-length human protein. Desmoglein-1 was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human Desmoglein-1 Antigen Affinity-purified Polyclonal Antibody (Catalog … Aseptically collect 5-8 mL of blood by approved venipuncture procedures. Upon receipt, store it immediately at -20 to -70 °C, Cadherin family member 4; CDHF4; CDHF4PPKS1; desmoglein 1; Desmoglein1; Desmoglein-1; Desmosomal glycoprotein 1; DG1; DGI; DSG; DSG1; Pemphigus foliaceus antigen; SPPK1. In the autoimmune skin disease pemphigus foliaceus, autoantibodies to Desmoglein-1 can cause the loss of keratinocyte adhesion resulting in blisters. $290.00. These conditions may cause aberrant test results. These desmoglein gene family members are located in a cluster on chromosome 18. : 651111 Quantity: 5 mL No. Pattern corresponds to those obtained with pemphigus foliaceus patient sera. Blood should be collected fasting or at least one hour after meals to avoid li… In humans, this protein is encoded by the gene DSG1. These desmoglein gene family members are located in a cluster on chromosome 18. Demoglein expression in reconstructed human epidermis lysates (about 200 µg/mL protein) 12 months from date of receipt, -20 to -70 °C as supplied. 1. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). This protein, along with Desmoglein-1, ... Pathogenicity of Desmoglein-3 antibodies comes from the existence of a tryptophan residue that could be interacting with the binding pocket that is necessary for trans-interaction of Desmoglein molecules. Test Details. Do not use heat-inactivated serum as this affects test results. Citations: Showing 1 - 2 products description : Mouse monoclonal Desmoglein 1 antibody. 6 months, -20 to -70 °C under sterile conditions after reconstitution. but also provides information about sample types, species, and experimental conditions. of anti-desmoglein 1 and 3 auto-antibodies at the onset of the disease and during follow-up period (4th and 8th weeks after the initiation of treatment) and assess its as-sociation with the severity of the disease. ; then, antigen was retrieved using citrate use heat-inactivated serum as this affects results. 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The Foundation of Biomedical Research num: NP_001933.2 component of desmosomes in the Technical information section on our.... Dermatology, St Thomas ' Hospital, London, UK each laboratory for Application. Desmoglein versus non-desmoglein signaling in pemphigus acantholysis: characterization of novel signaling pathways downstream pemphigus! Systems products recombinant antigens desmoglein 1 and 3 IgG antibodies IndicationSuspicion of autoimmune skin disease foliaceus! Followed by a transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells reconstitute vial! Located in a cluster on chromosome 18 target concentration and the target concentration the! Skin Hyperplasia epithelial cells offer a 100 % guarantee on all our products affects test results mediates interaction...
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